上海11选5走势图

新闻动态上海11选5走势图 > 新闻动态 > 苏州创谱教您液相色谱仪使用遇到的问题及对策
苏州创谱教您液相色谱仪使用遇到的问题及对策
点击次数:427 发布时间:2018-03-08 返回

上海11选5走势图1YEXIANGSEPUYIDEJIBENGONGZUOYUANLI

上海11选5走势图YEXIANGSEPUYISHIYOURONGYEZHUQI、GAOYABENG、JINYANGXITONG、SEPUFENLIZHU、JIANCEQIHESHUJUCHULIXITONGJIBUFENZUCHENG。GAOYABENGCONGRONGYEZHUQIZHONGCHOUZOULIUDONGXIANG,LIUJINGZHENGGEYIQIXITONG,XINGCHENGMIBIDEYETILIULU。YANGPINTONGGUOJINYANGXITONGZHURUSEPUFENLIZHU,ZAIZHUNEIJINXINGFENLI。ZHULIUCHUYEJINRUJIANCEQI,SHIYIBEIFENLIDEZUFENZHUYIBEIJIANCEQISHOUJI,BINGJIANGXIANGYINGZHIZHUANBIANWEIDIANXINHAOHOUJINGFANGDABEISHUJUCHULIXITONGJILUSEPUFENG,TONGGUOSHUJUCHULIXITONGDUIJILUDEFENGZHIJINXINGCUNCHUHEJISUAN。

2SHUYEBENGDECHANGJIANGUZHANGJIDUICE

上海11选5走势图SHUYEBENG/GAOYABENGSHIBAOZHENGHPLCXITONGLIULUCHANGTONG、LIULIANGHEYALIWENDINGDEZHONGYAOYIQIBUJIAN,MUQIANDUOYONGWANGFUSHIHENGLIUZHUSAIBENG,ZHUYAOYOUZHUSAIGAN、MIFENGDIANQUANHELIANGGEDANXIANGFAZUCHENG,YONGMADADAIDONGTULUNQUDONGZHUSAIGANZUOXIYEHEPAIYEDEWANGFUYUNDONG。CHANGFENWEIDANZHUSAIBENG、BINGLIANZHUSAIBENG、CHUANLIANZHUSAIBENGHEZHUSAIGEMOBENGDENGLEIXING。LIUDONGXIANGHUNHEFANGSHIFENWEIDIYAHUNHEHEGAOYAHUNHE。CHANGJIANBENGDEGUZHANGZHUYAOYOUYIXIAJIZHONG,BINGTIGONGJIEJUEDEBANFA。

2.1DANXIANGFAGUZHANG

 YOUYUQIUYUFAZUOMIFENGBUYAN,YETIDAOLIU,ZAOCHENGYALIBUWEN,SHENZHIQIUYUFAZUOZHANZAIYIQIZUSI。MIFENGBUYAN

上海11选5走势图ZHUYAOSHIWURANHUOQIPAOYINQI,QIUYUFAZUOZHANLIANYESHIYOUYUWURANHEMOSUNZAOCHENG。WEIBIMIANDANXIANGFAZHONGDEBAOSHIQIUHEFAZUOBEIWURAN,LIUDONGXIANGYINGSHIYONGHPLCJIDERONGJI,BINGQIEPEIHAODELIUDONGXIANGYIDINGYAOYONG0.45uLVMOGUOLVHETUOQI。RUGUOBENGBEIWEILIWURAN,KEFENBIEYONGSHUI、JIACHUN、YIBINGCHUN、ERLVJIAWANYICICHONGXI。CHONGXISHIYINGDAKAIXIEYEFA,zuiHOUZAIYONGSUOYONGDERONGYECHONGXIZHENGGEXITONG。QIPAOJINRUFAZHONGHUIJINTIEZAIFATIDEYICE,SHIBAOSHIQIUNANFANHUIDAOFAZUO,YINQIDAOLIU,BENGDEYALIHELIUSUHUIMINGXIANGAIBIAN。CISHI,YINGLIJIDAKAIXIEYEFA,DALIULIANG(2ml/min)CHONGXIBENG,ZHIZHIPAICHUYEWEIZHIXIANXING。YINWEIJIACHUNKERUNSHIBENGNEIBI,JICHUQIPAO,GUYEKESHIYONGTUOGUOQIDEJIACHUNCHONGXIBENG。ZHANGQIBUYONGDEBENGHUOXINZHUANGDEBENGTOUYINGGAIYONGJIACHUNGANQIPAO。BENGJINQIPAOYEKEZAIBENGTOUSHANGPAIQIPAO,JIYONGBANSHOUGUDINGZHUJINQIPAODEBENGTOU,NINGKAISHUCHUGUANDAODEYAMAO,SHOUDONGBENGSONGYE,KEJIANDAOQIPAOCONGKONGZHONGJIYACHULAI,ZHIZHIWUQIPAOPAICHUJIANGYAMAONINGJIN

[3]CAIQUSHANGSHUBANFARENGBUNENGSHIYALIWENDING,YINGKAOLVSHIFOUMIFENGDIANHUAILE?HUODANXIANGFAMOSUN、QIUBUGUANGHUADENG,XUGENGHUANBUJIAN。

2.2BENGDIANQUANGUZHANGBENGDIANQUANCHANGJIANDEGUZHANGSHISHENLOUHEDIANQUANSHOUSUNWURANXITONG。

上海11选5走势图YEXIANGSEPUXITONGYIBANQINGKUANGXIABUYAOSHIYONGQIANGSUANQIANGJIANRONGYE,YINWEIZHEIHUISUNHUAIMIFENGDIANHEZHUSAIGAN。MIFENGDIANQUANYUYUNDONGZHEDEZHUSAIGANJINMIJIECHU,KESHIZHUSAIZAIBENGTOUZIYOUYUNDONGERLIUDONGXIANGBULOUCHULAI,TASHIYEXIANGSEPUXITONGZHONGzuiYIMOSUNDEBUJIAN。YOUTIAOJIANDESHIYANSHIKESANGEYUEGENGHUANYICIDIANQUAN,FANGZHIBENGXITONGWURAN。RUGUOYONGHUANCHONGYANZUOLIUDONGXIANGZEGENGHUIJIASUDIANQUANDEMOSUN,YINGJISHICHONGXI。DANGDIANQUANSUNHUAISHI,BIAOXIANWEI:BENGYALIBUWEN、BENGTOULOUYE。YIDANCHUXIANZHEIGEXIANXIANG,YINGJINKUAIGENGHUANXINDIANQUAN。

上海11选5走势图WEIBIMIANSHANGSHUGUZHANGDEFASHENG,WOMENZAIFENXIGONGZUOZHONGYINGZHUYIYIXIAJIDIAN:

上海11选5走势图①SHIYONGCHAOCHUNSHUI(18MΩcm)、CHUNDUJIBIEJIAOGAODESHIJIHESEPUJIRONGJIPEIZHILIUDONGXIANG;

上海11选5走势图②PEIHAODELIUDONGXIANGYIDINGYAOCHOULVTUOQI,JIBIANYIQIYOUZAIXIANTUOQIZHUANGZHI,YEzuiHAOZAISHANGJIQIANJINXINGCHOULV。ZHENKONGCHOULVJIGUOLVKELIYETUOLEQIPAO,FEICHANGYOUXIAO。

③BENGZAIQIDONGQIANYIDINGYAOTONGGUOXIEYEFACHOUJINGBENGLIDEKONGQI。

上海11选5走势图④YONGHUANCHONGYANXITUOSHI,FENXIJIESHUHOUYIDINGYAOYONGSHUICHONGXIBENGHEZHENGGELIULUXITONG,BUYAORANGFUSHIXINGRONGYEZHILIUBENGZHONG,zuiHOUYONGYOUJIRONGJICHONGMANXITONG。

3.SEPUFENLIZHUDESHIYONGYUWEIHUZAIQIANSHUYIQIGONGZUOYUANLIBUFEN,WOMENYIJINGZHICHU,YEXIANGSEPUYIDEFENLISHIZAISEPUFENLIZHUZHONGSHIXIANDE。WOMENMUQIANGONGZUOZHONGDUOCAIYONGFANXIANGSEPUZHU,RUHEBAOHUHAOFENLIZHUJIANSHAOZHUDEWURANSHIYANZHANGZHUSHOUMINGDEGUANJIAN。

3.1怎样维护好色谱分离柱加保护柱(预柱)是保护分离柱的有效办法。保护柱是根内装填料与分离柱性质相近的短柱,接在分离柱之前,或代替流路过滤器。保护柱的作用是收集和阻塞分离柱口的化学垃圾,这些垃圾如果直接进入分离柱会逐渐堆积在柱头,zui终降低柱效能。保护柱是消耗品,如分析血浆样品,一般分析50个样品后需更换新柱芯。避免高压冲击分离柱。一般色谱柱都能承受得起高压,但经不住突然变化的高压冲击,这将改变柱床体积,影响柱效。引起高压冲击的原因主要有:进样器的样品阀的缓慢转动、泵启动太快、柱切换等操作。用手动进样阀时的压力变化不大,自动进样阀由于切换较慢,可能会造成压力冲击。
合理选择分离柱。我们知道,选择色谱分离柱主要根据两点,一是根据待测组分的分子量的大小;二是根据流动相条件,包括PH值、离子强度和溶剂极性等。传统硅胶基质的键合相柱要求流动相PH值在3-7之间,极端PH值(>8)的流动相能溶解硅胶,使键合相流失。而目前利用先进的杂化颗粒技术研制的高纯硅胶颗粒填料(如Waters公司的XTerra色谱柱),集硅胶和多孔聚合物填料的优点为一体,使有机硅烷单元取代了相当一部分占据颗粒内部和表面位置的硅羟基,使这种新型填料具有分辨率高、稳定性强、PH范围宽(1-12)的优势,其性能突破了传统液相色谱柱的极限。所以一定要根据分析的对象、流动相的条件选择合适的分离柱,茫然时可向公司的技术人员咨询。定期冲洗分离柱。每次分析工作结束时,冲洗完缓冲盐后,zui后要过度到用强溶剂冲洗柱子,如可用色谱纯甲醇、乙腈冲洗反相C18柱,目的是冲去吸附在柱上的强保留组分。如果用甲醇/水为流动相也应这样冲洗柱子,在一定程度上可恢复柱效。

3.2ZENYANGJIANSHAOZHUDEWURAN?zuiYOUXIAODEBANFASHICHUNHUAYANGPIN。WOMENZAIDUONIANDEFENXIGONGZUOZHONGFAXIAN,FANSHIYANGPINCHUNHUADEYUEGANJING,FENLIZHUDESHOUMINGJIUYUEZHANG;FANZHI,ZHIYOUTIQUMEIYOUCHUNHUADEYANGPIN,FENLIZHUZHUXIAOXIAJIANGJIAOKUAI。JIANLIYIGELIXIANGDESEPUFENXIFANGFAYINGGAIYOUYITAOYOUXIAODEYANGPINQIANCHULIFANGFA,TEBIESHIZAIFENXIXUEJIANGHENIAOYEYANGPINSHI,YAOTEBIEZHUYIYANGPINDETIQUYUCHUNHUA,JINLIANGSHICHULIGUODEYANGPINZHONGZAZHI(RUDAFENZIDANBAI、YOUJIWUDENG)JIANGDAOzuiDI,BAOHUZHUTOUHEZHUZI。TIQUCHUNHUAHAODEYANGPINzuiHAOYONGLIUDONGXIANGLAIRONGJIE,YIFANGMIANJIANSHAOSEPUTUZHONGDERONGJIFENGGANRAO,LINGYIFANGMIANYESHIJIANCHAYANGPINZAILIUDONGXIANGZHONGDERONGJIEXING,RUGUOYANGPINJINYANGHOUZAILIUDONGXIANGZHONGCHENDIAN,HUIDUSAIJINYANGQIHEZHUTOU,SHENZHIYANGPINFENJIE,CHUXIANGUAIFENG。RUGUOCHUXIANYANGPINYULIUDONGXIANGBURONGYAOSHEFAGAIBIANRONGJIETIAOJIAN,RUGENGHUANYANGPINRONGJI,HUOGAIJINCHULIYANGPINDEFANGFA,HUOGUOLV,QUCHUBURONGXINGWUZHI。

4JIANCEQIDECHANGJIANGUZHANGJIDUICE

上海11选5走势图YEXIANGSEPUYICHANGYONGDEJIANCEQIZHUYAOYOUZIWAI、YINGGUANGHEDIANHUAXUEJIANCEQI。ZHEIXIEJIANCEQIDEZUOYONGJIUSHISHOUJILIUJINGSEPUFENLIZHUDEGEZUFENZAIJIANCEQIZHONGDEXINHAO,GENJUZUFENGUANGXISHOUZHI/YINGGUANGQIANGDU/DIANJIBIAOMIANDIANLIUDEBIANHUAJISUANCHUZUFENDENONGDU

[4]。GEJIANCEQICHANGJIANGUZHANGZHUYAOYOU:

4.1JIXIANZAOYINJIAODAHEDUICE

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BUJINJIXIANHUICHUXIANJIANFENG,HAIHUIYINGXIANGJIANCE。YINCI,ZUODIANHUAXUEJIANCESHI,LIUDONGXIANGDEPEIZHIYAOHENYANGE,SHUIYAOCHAOCHUN、CHUHAIYUANWU,PEIHAOHOUYIDINGYAOGUOLVTUOQI,HAIYINGXIANYONGXIANPEI。RUGUOBENGXITONGBUSHIPKCAILIAO,JIDIANHUAXUEFENXIZHUANYONGYIQI,ERSHIPUTONGDEYEXIANGSEPUBUXIUGANGBENG,YINGDUIXITONGZHONGDEBUXIUGANGCAILIAODESHUYEBENG、JINYANGQIHEGUANDAO,ZAIFENXIQIANYONG6mol/LXIAOSUANRONGYEDUNHUA(ZHUYIDUANKAIFENLIZHU),KESUODUANJIXIANPINGHENGSHIJIAN。HAIKEZAILIUDONGXIANGZHONGJIARUEDTALIZIYINBIJI,YESHIKEYIDE。RUGUOYOUJIAODADEQIPAOJINDAOJIANCEQIZHONG,GUANGKAOBENGCHONGYOUSHITAIMAN,KEZANSHIJIANGBENGTINGZHI,GUANSHANGDIANHUAXUEJIANCEQI,CHAIXIAGONGZUODIANJI,YONGCHAOCHUNSHUICHONGXIDIANJIBIAOMIAN,YEHENZOUXIAODE,DANDUIYEXIANGSEPUJISHUBUTAISHULIANDEJISHUYUANYAOXIAOXINCAOZUO,BUYIFANFUZHEIYANGCHAIXIE。

4.2JIANCEQIWURANJIDUICE

上海11选5走势图GUANDAOWURANZUSAISHIJIANCEQICHANGJIANGUZHANG。DANGLIUDONGXIANGYONGLEHUANCHONGYANGENGHUANLIUDONGXIANGYOUBUDANGSHI,CHANGHUIZAIJIANCEQIGUANLUZHONGCHANSHENGCHENDIAN,KEXIANYONG5BEIZHUJIDESHUICHONGXI,ZAIYONG10BEIZHUJIDEQIANGRONGJI(RUYIJING)LIUGUO,ZAIYONG50BEIDEZHUTIJIDEYIBINGCHUNCHONGXI,zuiHOUHUANSHANGFANXIANGLIUDONGXIANGPINGHENG。DANGJIANCEQIGUANLUDUSAISHI,KEBIAOXIANWEIZHUYASHENGGAO,CISHI,YINGHEZHUTOUDUSAIXIANGQUFEN。KEDUANKAIZHUZI,ZHIJIEJIEJIANCEQI,RUBENGYARENGJIAOGAO,KEPANDUANYALISHENGGAOSHIYOUYUJIANCEQIGUANDAODUSAIZAOCHENG。JIANCEQIJINKOUSHICHANGFASHENGZUSAIDEDIFANG,ZHENGXIANGCHONGXIXIAOGUOCHANGBUMINGXIAN,WOMENDEJINGYANSHIJIANGJIANCEQIJINCHUKOUGUANDAODUIDIAO,YONG6mol/LXIAOSUANRONGYEFANCHONGJIANCEQI,NENGHENKUAIJIANGZUSAICHONGKAI,DANYAOZHUYIYONGDILIUSUCHONGXI,GUANCHAYALIBIANHUA。JIANCECHIZUSAIHEWURANDUOJIANZIWAIJIANCEQI。CHIZUSAIYEKEYONGSHANGSHUFANGFAFANCHONG,YIBANDOUNENGSHUTONG。TONGCHANG,CHIZUSAIDEGUZHANGBIJIAOSHAOJIAN,ERCHIWURANCHANGFASHENG,RUBUGANJINGDEYANGPINZAICHICHUANGSHANGJIJUDENG,DOUHUIZAOCHENGJIANCECHIWURAN。BIAOXIANWEIJIXIANZAOYINZENGDA,YIQILINGMINDUXIAJIANG。KEYONGZHUSHEQIYICIHUICHOUYIBINGCHUN、6mol/LXIAOSUAN,YAOXIAOXINCAOZUO,JIANGCHIZHONGDEWUWUCHONGXIGANJING,zuiHOUYONG100mlCHUNSHUIZHENGXIANGCHONGXIJIANCECHI。FANXIANGCHONGXIHAIKEFANGZHICHISHENLOU。DUIYUDIANHUAXUEJIANCEQI,YAOZHUYIWEIHUGONGZUODIANJIBIAOMIANDEQINGJIEDU,DANGJIANCEQIJIANCELEDALIANGJIAOZANGDEYANGPIN(RUXUEJIANG、NIAOYE)HUOYONGDEGUOJIU,YINGYONGHUAXUEFAHUOWULIPAOGUANGFADUIDIANJIBIAOMIANJINXINGQINGJIE,KETIGAOYIDINGDEJIANCELINGMINDU,DANYAOZHUYI,zuiHOUYIDINGYAOYONGCHUNSHUICHONGXIGANJING,BUYAODAILAIQITAWURAN。

上海11选5走势图WOMENJINJIUYOUXIANDEPIANFUTANLEYIDIANCONGSHIYEXIANGSEPUFENXIGONGZUODEJINGYAN,HAIYOUXUDUOWENTIXUYAOWOMENDAJIAGONGTONGJIAOLIUHETANTAO。YEXIANGSEPUYISHIXIANDAIKEXUEYANJIUZHONGGUANGFANSHIYONGDEFENXIYIQI,DANWOMENBIXUXUEHUIZHENGQUEDESHIYONGHEWEIHU,CAINENGGENGHAODEJIAYUTA,RANGHPLCJISHUZAIYIXUELINGYUFAHUIGENGDADEZUOYONG。

YEXIANGSEPUYIDEJIGESHIYONGWENTI

1. SEPUZHUZHONGDELIUDONGXIANGHUIPAIGANMA?

BUSHAOZUOSEPUFENLISHIYANDERENYUDAOGUOZHEIYANGDEQINGXING:BUSHENWEIJISHIBUCHONGLIUDONGXIANG,BENGJIANGRONGJIPINGZHONGDELIUDONGXIANGXIGANLE,HPLCXITONGYOUCIERTINGZHIGONGZUOLE。RUCIQINGKUANGSHIFOUHUISUNHUAISEPUZHU?BENGSHIFOUYIJIANGSEPUZHUZHONGSUOYOULIUDONGXIANGDOUPAIGANLE?SEPUZHUHAINENGSHIYONGMA?SHISHISHANG,RUGUOBENGJIANGRONGJIPINGZHONGDELIUDONGXIANGXIGAN,BINGBUHUIZAOCHENGSEPUZHUDESUNHUAI。JISHIBENGZHONGCHONGMANLEKONGQI,BENGYEBUHUIJIANGKONGQIPAIRUSEPUZHU。YINWEIBENGZHINENGSHUSONGYETI,ERBUNENGSHUSONGKONGQI。XIANGBIZHIXIA,LINGYIGEGENGKENENGFASHENGDEQINGKUANGSHIWANGJIGAISHANGSEPUZHULIANGDUANDEMIFENGGAIHUOGAIZITAISONGERSHISEPUZHUBIANGAN。TONGYANG,ZHENGGESEPUZHUGANHEDEQINGKUANGBUTAIRONGYIFASHENG,DUOBANKENENGZHISHISEPUZHULIANGDUANDEJIGEHAOMIBIANGANLE,YINHUIFADIAOSUOYOURONGJISHISEPUZHUBIANGANXUYAOXIANGDANGZHANGDESHIJIAN。JISHISEPUZHUZHENDEBIANGANLE,YEBUYIDINGJIUBUKEJIUYAOLE。KEYICHANGSHIYONGYIZHONGWANQUANTUOQIDE、BIAOMIANZHANGLIDIDERONGJI(RUJINGHAIQITUOQIDEJIACHUN)CHONGXISEPUZHUYICHUQUQITI。

JIAODIDEBIAOMIANZHANGLIYOUZHUYUJINRUNTIANLIAOBIAOMIAN;YITUOQIDERONGJIYINGGAINENGGOURONGJIEBINGQUCHUZHILIUZAITIANLIAOZHONGDEQITI。SEPUZHUDAYUEXUYAO(YI1mL/minDELIUSU)CHONGYIGEXIAOSHIHUOGENGDUODESHIJIANBEICHEDIJINRUN,HUIFUDAOZHENGCHANGZHUANGTAI。

上海11选5走势图2. SHIYONGPEEK(polyetheretherketone)GUANLUHEJIETOUXUYAOZHUYISHENMEWENTI?

RUGUOJINGCHANGXUYAOGAIBIANLIULUHUOGENGHUANBUTONGPINPAIDESEPUZHU,SHIYONGPEEKCAILIAOZHICHENGDEGUANLUHEJIETOUHUIFEICHANGFANGBIAN。PEEKGUANLURONGYILIANJIE;PEEKJIETOUBUJINWUXUGONGJU,SHOUNINGJIKEGUDING,ERQIERONGYIDIAOJIEZHUIGUZHIWAIDEGUANLUZHANGDU,FANGBIANYUBUTONGPINPAIHUOGUIGEDESEPUZHUXIANGLIANJIE。SHIYONGCILEICAILIAODEGUANLUXUYAOZHUYIDESHI:PEEKDUILUDAIWANTINGHESIQINGFUNANDEJIANRONGXINGBUHAO。SUIRANWEIGUANCHADAOSHANGSHURONGJIRONGJIEPEEKCAILIAODEMINGXIANJIXIANG,DANPEEKYUDAOSHANGSHURONGJIHUIBIANCUI。LINGYIGEXIYAOKAOLVDEYINSUSHIYALIXIAN。BUXIUGANGGUANKENAISHOU6000psiDEYALI,DANPEEKGUANZHINENGNAISHOUJIN4000psi(DANDUOSHUHPLCYINGYONGXITONGYALIBUHUICHAOGUO3000psi)。SHIYONGPEEKJIETOUSHIZEWUXUDANXINJIETOUNAIRONGJIXINGNENG,YINWEIJIETOUJIHUHUOHENSHAOYURONGJIZHIJIEJIECHU。DANSHOUNINGGUDINGDEPEEKJIETOUYALIXIANDIYUBUXIUGANGGUAN,YINERYALITAIGAOSHI,KENENGHUISHIJIETOUZAIGUANLUSHANGHUADONGERCHANSHENGSITIJIHUOLOUYE。

3. 如何预防液相泵的故障:
要保持泵的良好操作性能,必须维护系统的清洁保证溶剂和试剂的质量对流动相进行过滤和脱气


下面列出预防泵故障的几项措施: 

1).YONGGAOZHILIANGSHIJIHEHPLCJIRONGJI;

2)、GUOLVLIUDONGXIANGHERONGJI;

3)、TUOQI;

4)、MEITIANKAISHISHIYONGSHIFANGKONGPAIQI,GONGZUOJIESHUHOUCONGBENGZHONGXIQUHUANCHONGYE;

5)、BURANGSHUIHUOFUSHI

6)、DINGQIGENGHUANDIANQUAN;

上海11选5走势图7)、XUYAOSHIJIARUNHUAYOU;

8)、查阅有关泵操作手册中的其它建议。
处于良好操作状态的泵,应该能使色谱图上的基线平稳;保留时间的重复性好。在等度洗脱时压力波动小于2%。梯度洗脱时压力变化应是缓慢和平稳的。为了使故障发生后尽快排除,平时应常备密封、单向阀(入口与出口)、泵头装置、各式接头、保险丝等部件,以及更换工具。

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